Payloads
RNPs delivery
Ribonucleoprotein (RNP) delivery is a genome engineering approach in which pre-assembled CRISPR-Cas9 complexes are directly introduced into cells to generate targeted gene knockout (KO).
Following delivery, the RNP complex creates a site-specific DNA break that is repaired by the cell, often resulting in disruption of the target gene. Because the editing machinery is delivered as a pre-formed complex and rapidly degraded after use, RNP delivery provides a transient “hit-and-run” editing profile with reduced risk of off-target effects. As no DNA integration is required, RNP-mediated gene knockout has become a powerful tool for functional genomics, disease modeling, target validation, and cell engineering.
High Editing Efficiency with LumiPore® Photoporation
Coupled with LumiPore photoporation, researchers can efficiently deliver CRISPR-Cas9 RNPs into sensitive and hard-to-transfect cell types while maintaining high cell viability and functionality, enabling the generation of high-quality edited cells.
Validation & Application Datasets
The following section highlights representative examples of CRISPR-Cas9 RNP delivery and gene KO achieved with the LumiPore platform in both cell lines and primary cells. The presented data have been generated and validated in collaboration with research partners and customers, demonstrating the versatility of the technology across diverse biological systems. Additional data, validated protocols, and info on specific applications can be found in the corresponding sections.
High-Throughput Transfection.Automation-Ready.
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